Fig. 1

Differentially gene expression in the mummichog midbrain/diencephalon in response to injury at 1 h post-lesion. a Placement of the mechanical lesion to the mummichog midbrain/diencephalon. Abbreviations. cb = cerebellum, fb = forebrain, hb = hindbrain, mb = midbrain, sc = spinal cord. b Frontal sections of mummichog midbrain/diencephalon with Nissl stain (right; magnification = 25X) and neuroanatomical areas in a mirror image schematic (left). Boxes show regional intact (left column) vs lesioned (right column) tissue (magnification = 160X). Abbreviations: dhypl = diffuse nucleus of the inferior hypothalamic lobe, lr = lateral recess, nmlf = nucleus of the medial longitudinal fascicle, nr = nuclear ruber, ot = optic tectum, tl = torus longitudinalis, ts = torus semicircularis, vc = valvula cerebelli. c MA plot of the distribution of expressed genes at 1 h post-injury as determined by RNA-seq. Red dots indicate DEGs with an FDR of q ≤ 0.1 (181 genes) or ≤ 0.4 (223 genes). d, e, f Upregulation of DEGs, pim-2-like (q = 5.09E-46) (d), syndecan-4-like (q = 5.22E-30) (e), & cd83 (q = 2.97E-23) (f) at 1 h post-lesion (One-Way ANOVA; p < 0.001 for each. Tukey’s Post Test; *** = p < 0.0001, ** = p < 0.001, * = p < 0.05. n = 10 for each). g Validation of a non-differentially expressed gene, brain igf-1 (n = 8)