Fig. 1

Spatiotemporal expression of TSK in the inner ear. a, b At E9.5 and E11.5, TSK-expressing cells were localized in the ventral tissues of the otocyst, which would differentiate into the cochlear duct (black arrowheads). c-h At E13.5 and E15.5, TSK-expressing cells were localized at the LW of the cochlear duct, which would differentiate into SV (asterisks in c, d, f, g), and not SGCs (e, h). i-k At P0, TSK was expressed in some SGCs (white arrowheads in i, k,), the OC (dagger in i, j), LW, which differentiates into the SV and SLi (asterisks in i, j), and in the GER, which differentiates into the spiral limbus and tectorial membrane (double dagger in i, j). l-n At P10, TSK was expressed in some SGCs (white arrowheads in l, n), the OC (dagger in l, m), the SV (double asterisk in l, m), and type I fibrocytes of the SLi (triple asterisk in l, m). (o-q) At P30, TSK was expressed in some SGCs (white arrowheads in o, q), the OC (dagger in o, p), the SV (double asterisk in o, p), and type III fibrocytes of the SLi (quattro asterisk in o, p). GER: greater epithelial ridge, LW: lateral wall, OC: organ of Corti, SGC: spiral ganglion cell, SV: stria vascularis, SLi: spiral ligament. Scale bars represent 100 μm (a and b) or 50 μm (c-q)