Fig. 5

Assessment of spiral ganglion cells in the CONT and TSK-KO mice. a Gross morphology. b Differences in numbers were not significant at apical (p = 0.39) and middle (p = 0.15) turns, but were significant at basal turns (*, p = 0.02). c Immunolabeling showed that Sox2 accumulated in the nuclei of the spiral ganglion cells in the CONT mice but was suppressed in the TSK-KO mice at E13.5, E15.5, and P0. Sox2 translocated to the cytosol at P0 in the TSK-KO mice. (C′) Magnified view of the white hollow square in C. d qRT-PCR for Sox2 mRNA in the spiral ganglion tissue collected by laser microdissection did not detect significant differences in Sox2 mRNA between the CONT and TSK-KO mice. p = 0.09 at E13.5; p = 0.34 at E15.5; p = 0.21 at P0. N.S.: not significant