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Fig. 4 | Molecular Brain

Fig. 4

From: A rare CACNA1H variant associated with amyotrophic lateral sclerosis causes complete loss of Cav3.2 T-type channel activity

Fig. 4

Electrophysiological characterization of Cav3.2 WT and ΔI153 expressed in trans. a Representative T-type current traces recorded from tsA-201 cells expressing WT channels in combination with either the ΔI153 variant (WT:ΔI153, red traces) or the cation-impermeant but trafficking-competent Cav3.2 pore mutant (WT:PM; grey traces) in a ratio 1:1. b Corresponding mean current-voltage relationship (I/V) for WT:ΔI153 (black|red circles), and WT:PM (black|grey circles) conditions. c Corresponding mean maximal macroscopic conductance (Gmax). d Co-immunoprecipitation of Cav3.2 from tsA-201 cells co-transfected with a Myc-tagged and GFP-tagged Cav3.2. The left panel shows the result of the co-immunoprecipitation of Myc-Cav3.2 with GFP-Cav3.2 using an anti-Myc antibody. The middle and right panels show the immunoblot of GFP-Cav3.2 and Myc-Cav3.2 using and anti-GFP and anti-Myc antibody, respectively

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