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Table 2 Comparison of viral tracers of anterograde trans-mulisynaptic tracing

From: A mutant vesicular stomatitis virus with reduced cytotoxicity and enhanced anterograde trans-synaptic efficiency

StrainGenomeDirCell toxicityDays of survivalExpressing levelOperation riskCre/flp dependent
WT-VSV-ss RNA
12 kb
AHigh3–5HighLowN
VSV-N(R7A)-ss RNA
12 kb
AMedium10–15HighLowN
HSV H129ds DNA
153 kb
A/RHigh3–5MediumMediumY
HSV H8ds DNA
153 kb
A/RHigh3–5HighMediumY
  1. Abbreviations: -ss RNA Negative-strand, single strand RNA, ds DNA Double strand DNA, Dir Direction of transsynaptic tracing, “A” Anterograde, “R” Retrograde, “N” Can’t be used as a cre/flp dependent anterograde trans-multisynaptic viral tracer, “Y” Can be used as a cre/flp dependent anterograde trans-multisynaptic viral tracer. VSV is a negative-stranded RNA virus, HSV is a double stranded DNA virus, there are many different characteristics between them, including genome, toxicity and application, etc. Researchers should choose the virus according to different experimental needs