Fig. 2

SENP2 cKO mice exhibited hyperactivity and decreased anxiety-like behavior. a Representative exploratory tracks of either littermate control (Ctrl) or cKO mice in the open field. b-i Analysis of open field exploration behavior for b distance traveled (m): increased locomotor activity of cKO mice at every 5 min block (5 min: Ctrl: 18.91 ± 1.03, n = 19; cKO: 31.69 ± 1.81, n = 12; p < 0.0001; 10 min: Ctrl: 15.76 ± 0.73, n = 19; cKO: 35.64 ± 2.22, n = 12; p < 0.0001; 15 min: Ctrl: 15.40 ± 0.96, n = 19; cKO: 37.76 ± 2.53, n = 12; p < 0.0001; 20 min: Ctrl: 14.60 ± 0.96, n = 19; cKO: 39.16 ± 3.13, n = 12; p < 0.0001; 25 min: Ctrl: 14.75 ± 0.88, n = 19; cKO: 36.61 ± 3.62, n = 12; p < 0.0001; 30 min: Ctrl: 15.24 ± 0.88, n = 19; cKO: 35.40 ± 3.92, n = 12; p < 0.0001; two-way ANOVA analysis followed by Bonferroni’s post-hoc), and c increased locomotor activity of cKO mice in the 30-min open field test (Ctrl: 94.66 ± 4.43, n = 19; cKO: 216.26 ± 15.81, n = 12; p < 0.0001, Welch’s t-test). d Analysis of number of entries in center area at every 5 min: Increased number of entries of cKO mice in center area at 10, 15 and 20 min (5 min: Ctrl: 12.16 ± 1.56, n = 19; cKO: 12.50 ± 1.73, n = 12; p > 0.9999; 10 min: Ctrl: 10.11 ± 1.11, n = 19; cKO: 15.92 ± 1.49, n = 12; p = 0.0422; 15 min: Ctrl: 10.53 ± 1.05, n = 19; cKO: 16.33 ± 1.65, n = 12; p = 0.0425; 20 min: Ctrl: 11.11 ± 1.35, n = 19; cKO: 18.33 ± 1.78, n = 12; p = 0.0052; 25 min: Ctrl: 11.58 ± 1.27, n = 19; cKO: 14.92 ± 1.45, n = 12; p = 0.7143; 30 min: Ctrl: 13.33 ± 1.35, n = 19; cKO: 16.75 ± 2.18, n = 12; p = 0.6865; two-way ANOVA analysis followed by Bonferroni’s post-hoc), and e increased number of entries of cKO mice in the 30-min open field test (Ctrl: 68.11 ± 6.00, n = 19; cKO: 94.75 ± 6.48, n = 12; p = 0.0068, Student’s t-test). f Analysis of duration spent (s) in the center of the field at every 5 min: An increased center time was observed in cKO mice at 10, 15 and 20 min (5 min: Ctrl: 14.44 ± 2.52, n = 19; cKO: 17.35 ± 3.57, n = 12; p > 0.9999; 10 min: Ctrl: 13.17 ± 2.30, n = 19; cKO: 24.00 ± 12.14, n = 12; p = 0.0477; 15 min: Ctrl: 14.37 ± 2.34, n = 19; cKO: 25.12 ± 3.00, n = 12; p = 0.0497; 20 min: Ctrl: 14.47 ± 2.19, n = 19; cKO: 33.24 ± 4.43, n = 12; p < 0.0001; 25 min: Ctrl: 17.20 ± 2.77, n = 19; cKO: 25.00 ± 3.07, n = 12; p = 0.3334; 30 min: Ctrl: 19.00 ± 2.37, n = 19; cKO: 24.40 ± 3.26, n = 12; p > 0.9999; two-way ANOVA analysis followed by Bonferroni’s post-hoc), and g increased time spent (s) of cKO mice in the center of the field in the 30-min open field test (Ctrl: 91.64 ± 9.97, n = 19; cKO: 149.04 ± 11.42, n = 12; p = 0.0009, Student’s t-test). h Analysis of distance traveled (m) in center area at every 5 min: Increased distance traveled of cKO mice in the center area at 10 and 20 min (5 min: Ctrl: 1.72 ± 0.26, n = 19; cKO: 1.94 ± 0.32, n = 12; p > 0.9999; 10 min: Ctrl: 1.44 ± 0.18, n = 19; cKO: 2.38 ± 0.18, n = 12; p = 0.0379; 15 min: Ctrl: 1.53 ± 0.17, n = 19; cKO: 2.31 ± 0.18, n = 12; p = 0.1428; 20 min: Ctrl: 1.61 ± 0.19, n = 19; cKO: 2.57 ± 0.33, n = 12; p = 0.0324; 25 min: Ctrl: 1.74 ± 0.19, n = 19; cKO: 2.26 ± 0.26, n = 12; p = 0.7768; 30 min: Ctrl: 1.88 ± 0.26, n = 19; cKO: 2.39 ± 0.32, n = 12; p > 0.9999; two-way ANOVA analysis followed by Bonferroni’s post-hoc), and i Increased distance traveled (m) in center area of the field in the 30-min open field test (Ctrl: 9.93 ± 0.95 m, n = 19; cKO: 13.85 ± 1.00 m, n = 12; p < 0.0107, Student’s t-test). j Representative track of exploration in elevated plus maze of either littermate control (Ctrl) or cKO mice. k, i Analysis of number of entries in open arms or closed arms: more number of cKO mice entries in open arms (Ctrl: 6.84 ± 0.97, n = 19; cKO: 21.58 ± 3.91, n = 12; p = 0.0031, Welch’s t-test), l less number of cKO mice entries in closed arms (Ctrl: 25.26 ± 1.32, n = 19; cKO: 19.00 ± 2.49, n = 12; p = 0.0213, Student’s t-test), m, n Analysis of percentage of time spent in open arms or closed arms: more percentage of time spent in closed arms of cKO mice (Ctrl: 6.85 ± 1.54%, n = 19; cKO: 37.20 ± 6.90%, n = 12; p = 0.0010, Welch’s t-test), n less percentage of time spent in closed arms of cKO mice (Ctrl: 68.31 ± 2.94%, n = 19; cKO: 43.36 ± 7.81%, n = 12; p = 0.0096, Welch’s t-test). o Novelty suppressed feeding. cKO mice have less latency (s) to feeding compared with littermate control mice (Ctrl: 294.79 ± 36.24, n = 19; cKO: 89.42 ± 38.73, n = 12; p = 0.0008, Student’s t-test). All data presented as mean ± S.E.M.