Fig. 1

AAV vector construction and validation of AD pathology in APP/PS1 mice. a Lysates from HEK293TN cells transfected with the indicated constructs were immunoblotted with anti-Flag (top) or actin (bottom) antibodies. Representative blots are shown (n = 3). Expression of a NT4-TAT-CBD3 protein was noted running at a higher molecular weight than the NT4-TAT protein devoid of the CBD3 fragment. b In contrast to wildtype mice, APP/PS1 mice (male 3–4 months old) exhibit typical amyloid-beta aggregation as revealed by staining with an antibody against Aβ-42. Higher magnification images of the micrographs of the CA1–2 transitional field of the hippocampus. Representative of n = 3 for age-matched control and n = 3 for the APP/PS1 mice. *P < 0.05, Mann & Whitney non-parametric test