Fig. 2

Transcriptome analyses in the post-stroke brain. a The MA plots indicate the differentially expressed genes (DEGs) in each comparison with control (C; sham) at day 1, 3, 7, 14, and 28 post-stroke (d1, d3, d7, d14, d28), respectively. DEG; differentially expressed gene. Up- (red) and down-regulated (blue) genes are dotted in each color. b The histogram shows the differentially expressed genes (DEGs) that were up- or down-regulated at each time point, respectively. c The heatmap shows the level of gene expression [in log10 (FPKM+ 1)] across the 6 temporal samples (C, d1, d3, d7, d14, d28) and categorizes them into 4 groups dependent on the gene expressing patterns in the dendrogram. Up- and down-regulated genes are colored in red and blue, respectively. The regions of different colors represent different clusters. FPKM, Fragments Per Kilobase of transcript per Million mapped reads. Control (C); sham-operated mice. d Primary component analysis (PCA) plot clustered 6 temporal samples (C, d1, d3, d7, d14, d28) into 4 categories (I ~ IV) dependent on principal component 1 (PC1, X-axis, 31.4%) and 2 (PC2, Y-axis, 22.6%)