Fig. 4
From: Axl receptor tyrosine kinase is a regulator of apolipoprotein E
Axl is the target of AZ7235 and plays a role in regulating apoE homeostasis. (a) Secreted apoE and (b) APOE mRNA levels were measured in WT and AXL−/− CCF-STTG1 cells after 72 h treatment with vehicle control DMSO, 1 μM T0901317 or 3 μM AZ7235. (c) Secreted apoE and (d) APOE mRNA were measured in CCF-STTG1 cells after transfection with siRNAs targeting AXL for 72 h. (e) Representative immunoblot of Axl after siRNA transfection. Graphs represent fold-change over scrambled siRNA control (dashed line) and +/− 95% confidence intervals from 3 independent experiments. (f) Representative immunoblot of Axl in WT and K567R Axl stable-expressing AXL−/− CCF-STTG1 cells. Immunoblot images were cropped to show relevant lanes. (g) Secreted apoE levels were measured in AXL−/− CCF-STTG1 stably transfected with empty vector (EV), WT or K567R Axl. Error bars represent standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to vehicle control using blocked ANOVA post-hoc tests. ### P < 0.001 comparing between WT vs. AXL-/- within each drug condition