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Fig. 1 | Molecular Brain

Fig. 1

From: 1-O-Acetylgeopyxin A, a derivative of a fungal metabolite, blocks tetrodotoxin-sensitive voltage-gated sodium, calcium channels and neuronal excitability which correlates with inhibition of neuropathic pain

Fig. 1

1-O-acetylgeopyxin A inhibits calcium influx via voltage-gated calcium channels in sensory neurons. a Differential interference contrast (DIC) and pseudocolored fluorescent images of DRG neurons treated with vehicle (1% DMSO), visualized for Fura2-AM before and after stimulations with KCl (40 and 90 mM) during Ca2+ imaging. Scale bar is 50 μm. b Average responses of sensory neurons incubated overnight with vehicle (control) or 20 μM of geopyxin A, 1-O-acetylmethylgeopyxin A, methylgeopyxin A, 1-O-acetylgeopyxin A and geopyxin C in response to 40 and 90 mM KCl (to trigger opening of low- and high voltage activated calcium channels) (n = 81 to 204 neurons from 3 separate rats). Incubation with 20 μM 1-O-acetylmethylgeopyxin A or methylgeopyxin A killed the cells. Asterisk indicate statistical significance compared with cells treated with vehicle (*P < 0.05, one-way ANOVA with Dunnett’s post hoc test). c chemical structure of 1-O-acetylgeopyxin A. Experimenter was blinded to the treatment condition. Unless otherwise noted, all data is mean ± S.E.M.

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Molecular Brain

ISSN: 1756-6606