Fig. 2

1-O-acetylgeopyxin A inhibits total Ca²⁺ currents in dorsal root ganglion (DRG) sensory neurons. a Representative traces of total Ca²⁺ currents from DRG sensory neurons treated with 0.1% DMSO (control) or 20 μM 1-O-acetylgeopyxin A. Currents were evoked by 200 ms pulse between − 70 and + 60 mV. b, c Summary of the normalized (pA/pF) total Ca²⁺ current density versus voltage relationship (b) and peak total Ca²⁺ current density at + 10 mV (mean ± SEM) (c) from DRG sensory neurons treated as indicated. (d, e) Boltzmann fits for normalized conductance, G/Gmax, voltage relations for voltage dependent activation (d) and inactivation (e) of sensory neurons treated as indicated. V_1/2 values for activation and inactivation are presented in Table 1. Asterisks indicate statistical significance compared with cells treated with 0.1% DMSO (*P < 0.05, unpaired two-tailed Student’s t test, n = 21 per condition)