Fig. 2

Dot1l-cKO causes loss of dorsal differentiation markers at E13.5 and extensive cell death at E18.5. (a) Nissl staining on prenatal (E18.5) lumbar spinal cord hemi-sections in control and Dot1l-cKO littermates. Black squares in the left panels refer to the higher magnifications, A’ and A” to the right. Within the right panels, boxed magnifications on top right refer to the smaller boxed areas showing pyknotic nuclei. Yellow arrowheads in panels to the right highlight pyknotic nuclei. (b, c) In situ hybridization (ISH) for Gad65 transcripts in lumbar spinal cord hemi-sections at E18.5 (b) and at E13.5 (c) in control and in Dot1l-cKO littermates. Higher magnifications in B′, B″, C′, C″. (d) ISH for Lhx5 transcripts in lumbar spinal cord hemi-sections at E13.5 of control and Dot1l-cKO. Higher magnifications in D’ and D”, scale as in C, C′. (e) ISH for Reln transcripts in lumbar spinal cord hemi-sections at E13.5 of control and Dot1l-cKO. Higher magnifications in E’ and E”, scale as in C, C′. Black squares: regions of interest described in the results, magnified next or below the figure of reference. Hemicord profiles highlighted by dotted black line. Scale bars: A: 200 μm; A’, B, B′, C, C′: 100 μm; inset in A’: 10 μm