Fig. 3

Autophagic degradation impaired by PPA (a) Representative western blot of p62 after PPA treatment. b Quantification of p62 levels with respect to GAPDH (n = 4). c Representative immunofluorescence images showing p62 staining in hippocampal neurons and PPA-treated cells. d Quantification of number of p62 puncta from images in (C) (n = 8). Bar graph showing mean ± SEM of representative groups. Scale bar is 10 μm. *p < 0.05, **p < 0.01