Fig. 2

Drp1 protein distribution in the cerebral cortex, cerebellum, hippocampal region and spinal cord. a. The distribution of Drp1 in the cerebral cortex. Drp1 was barely expressed in the first layer of the CTX, and specific staining results were obtained in the second/third layer. Compared with the other layers, Drp1 expression was higher in the fifth layer than the others. b. The distribution of Drp1 in the cerebellum. Drp1 was expressed in the cytoplasm around the nucleus (DAPI). In addition, Drp1-positive protuberant structures were also found in the mo region, which were long strips and, possibly, axons. c. The distribution of Drp1 in the hippocampal region. The distribution of Drp1 (green) and DAPI (blue) in the CA1 and DG regions. Drp1 was expressed at low levels in CA1, DG-mo and DG-sg. Drp1 was highly expressed in DG-po. d. The magnified image of C. From D, we can see that some Drp1-positive cells in DG-mo and DG-po regions had a protuberance around them, which might represent axons or dendrites. e. Drp1 distribution in the spinal cord. e provides a complete view of the spinal cord. In the deep layers (VII, VIII, IX, X), Drp1 expression was slightly higher than in the superficial layers (I, II, III). f. In the spinal dorsal horn, Drp1 was weakly expressed. Bar = 200 μm a, c, e; 100 μm d, f; 30 μm b. Abbreviations: CTX: cerebral cortex; cing: cingulum bundle; SIMpu: simple lobule, Purkinje cell; SIMmo: simple lobule, molecular layer; SIMgr: simple lobule, granular layer; cc: corpus callosum; CA: Ammon’s horn; CA1-so: field CA1, stratum oriens; CA1-sp: field CA1, pyramidal layer; CA1-sr: field CA1, stratum radiatum; CA1-slm: field CA1, stratum lacunosum-moleculare; DG-mo: molecule layer of dentate gyrus; DG-sg: granular cell layer of dentate gyrus; DG-po: polymorphic layer of dentate gyrus