Fig. 3

Different patterns of astrogliosis in the cortex and striatum. a, b Coronal sections were obtained from the intact or ATP (400 nmole)-injected cortex or striatum at the indicated times after injection, and immunostained for GFAP. Photographs of the most damaged sections in the injured brain are shown. *, injection sites; arrowheads and arrows, GFAP-positive cells in different brain regions; cc, corpus callosum; bv, blood vessel; v, lateral ventricle. c Process lengths of astrocytes adjacent to the damage core (circles) were analyzed 3 and 7 d post injury using MetaMorph as described in the Methods. For each time point, 4–5 animals were used. Values are means ± SEMs. n, approximately 150; *, p < 0.05; ns, not significant; two-way ANOVA, F(1,140) = 15.41, p = 0.0001, time; F(1,140) = 7.189, p = 0.0082, region. *, injection sites. d Sections obtained from the intact or ATP-injected cortex or striatum 1 and 15 d post injection, and immunostained for MAP2/GFAP or TH/GFAP. Dotted lines, borders of MAP2- and TH-positive neurites; *, injection sites