Antibodies | Dilution factors for | |
---|---|---|
Western blot | Immunostaining | |
Rabbit anti-ALDH1L1 (Abcam, ab87117) | 1:1000 | 1:100 |
Rabbit anti-GFAP (Neuromics, RA22101) | Â | 1:1000 |
Rabbit anti-Col1a1 (Novus, NB600–408) |  | 1:200 |
Rabbit anti-Ki67 (Chemicon, AB9260) | Â | 1:100 |
Mouse anti-GFAP (Sigma-Aldrich, G3893) | 1:5000 | 1:1000 |
Goat anti-GAPDH (Santa cruz, sc-48167) | 1:5000 | Â |
Rat anti-CD45 (Mybiosource, MBS520149) | Â | 1:1000 |
Rabbit anti-Iba-1 (Wako, 019–19741) |  | 1:1000 |
Rat anti-CD31 (BD Pharmingen, 550274) | Â | 1:50 |
Chicken anti-MAP2 (Abcam, ab5392) | Â | 1:500 |
Rabbit anti-TH (Pel Freez, P40101–0) |  | 1:1000 |
DAPI (Sigma-Aldrich, D9542) | Â | 1:2000 |
Goat anti-rabbit IgG HRP (Koma biotech, K0211708) | 1:10000 | Â |
Goat anti-mouse IgG HRP (Koma biotech, K0211589) | 1:10000 | Â |
Rabbit anti-Goat IgG HRP (Koma biotech, K0211303) | 1:10000 | Â |
Goat Anti-rabbit IgG (H + L) (Vector Laboratories, BA-1000) |  | 1:200 |
Goat Anti-rat IgG (H + L) (Vector Laboratories, BA-9401) |  | 1:200 |
Goat anti-mouse IgG, Alexa Fluor 488 (Thermo Fisher scientific, A-11029) | Â | 1:500 |
Goat anti-chicken IgY, Alexa Fluor 488 (Thermo Fisher scientific, A-11039) | Â | 1:500 |
Goat anti-rabbit IgG, Alexa Fluor 594 (Thermo Fisher scientific, A-11037) | Â | 1:500 |
Goat anti-rat IgG, Alexa Fluor 633 (Thermo Fisher scientific, A-21094) | Â | 1:500 |
Goat anti-mouse IgG, Alexa Fluor 568 (Thermo Fisher scientific, A-11004) | Â | 1:500 |