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Table 1 Antibodies used in this study

From: Region-specific astrogliosis: differential vessel formation contributes to different patterns of astrogliosis in the cortex and striatum

Antibodies

Dilution factors for

Western blot

Immunostaining

Rabbit anti-ALDH1L1 (Abcam, ab87117)

1:1000

1:100

Rabbit anti-GFAP (Neuromics, RA22101)

 

1:1000

Rabbit anti-Col1a1 (Novus, NB600–408)

 

1:200

Rabbit anti-Ki67 (Chemicon, AB9260)

 

1:100

Mouse anti-GFAP (Sigma-Aldrich, G3893)

1:5000

1:1000

Goat anti-GAPDH (Santa cruz, sc-48167)

1:5000

 

Rat anti-CD45 (Mybiosource, MBS520149)

 

1:1000

Rabbit anti-Iba-1 (Wako, 019–19741)

 

1:1000

Rat anti-CD31 (BD Pharmingen, 550274)

 

1:50

Chicken anti-MAP2 (Abcam, ab5392)

 

1:500

Rabbit anti-TH (Pel Freez, P40101–0)

 

1:1000

DAPI (Sigma-Aldrich, D9542)

 

1:2000

Goat anti-rabbit IgG HRP (Koma biotech, K0211708)

1:10000

 

Goat anti-mouse IgG HRP (Koma biotech, K0211589)

1:10000

 

Rabbit anti-Goat IgG HRP (Koma biotech, K0211303)

1:10000

 

Goat Anti-rabbit IgG (H + L) (Vector Laboratories, BA-1000)

 

1:200

Goat Anti-rat IgG (H + L) (Vector Laboratories, BA-9401)

 

1:200

Goat anti-mouse IgG, Alexa Fluor 488

(Thermo Fisher scientific, A-11029)

 

1:500

Goat anti-chicken IgY, Alexa Fluor 488

(Thermo Fisher scientific, A-11039)

 

1:500

Goat anti-rabbit IgG, Alexa Fluor 594

(Thermo Fisher scientific, A-11037)

 

1:500

Goat anti-rat IgG, Alexa Fluor 633

(Thermo Fisher scientific, A-21094)

 

1:500

Goat anti-mouse IgG, Alexa Fluor 568

(Thermo Fisher scientific, A-11004)

 

1:500