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Fig. 4 | Molecular Brain

Fig. 4

From: Conditional knockout of leptin receptor in neural stem cells leads to obesity in mice and affects neuronal differentiation in the hypothalamus early after birth

Fig. 4

Depletion of Lepr in nestin-expressing cells does not affect the fate of NPCs in the hypothalamus of adult mice. a Experimental scheme. Nes-cKO mice or control littermates received BrdU twice daily between P35 and P43 and were sacrificed at P70. b Representative images showing that BrdU-expressing cells colocalize with NeuN, GFAP, and OLIG2. Shown to the right is the quantification of the percentage of BrdU+ cells displaying co-localization with each cell type. Scale bar, 50 μm. c No significant change in the ratio of BrdU+NeuN+ cells to total BrdU+ cells in Nes-cKO mice compared with control littermates. Scale bar, 100 μm. d No significant change in the ratio of the BrdU+OLIG2+ cells to total BrdU+ cells in the Nes-cKO mice compared with control littermates (n = 4 mice per group). Scale bar, 100 μm. Student’s two-tailed, unpaired t-test. Error bars indicate s.e.m.

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Molecular Brain

ISSN: 1756-6606