Fig. 1
From: mGluR5 regulates REST/NRSF signaling through N-cadherin/β-catenin complex in Huntington’s disease
mGluR5 modulates N-cadherin phosphorylation but not β-catenin expression in primary neuronal cultures. Representative western blots and quantification of fold change of N-cadherin phosphorylation at Y860 (pY860) and β-catenin expression with the corresponding loading controls in primary cultured corticostriatal neurons derived from E15 wild-type mouse embryos stimulated with either the mGluR group I agonist DHPG (100 μM) (a and b) or the mGluR5-selective NAM, CTEP (10 μM) (c and d) for 10, 30, 60, 180 and 360 mins. N-cadherin-pY860 was normalized to total N-cadherin, and β-catenin was normalized to vinculin (n = 4–5 for each group). Values represent mean ± SEM and are expressed as a fraction of the non-treated (NT) cultures. * denotes P < 0.05 versus NT cultures. Statistical significance was assessed by one-way ANOVA and Fisher’s LSD multiple comparisons