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Fig. 1 | Molecular Brain

Fig. 1

From: D1 receptors in the anterior cingulate cortex modulate basal mechanical sensitivity threshold and glutamatergic synaptic transmission

Fig. 1

Specificity of CRISPR/Cas9 mediated somatic knockout of Drd1 in Striatum. a Drd1 targeting sequence and corresponding protospacer adjacent motif (PAM). b Evaluation of Drd1 targeting sgRNA by Tracking of indels by decomposition (TIDE) analysis in Neuro2A cells. c Schematic diagram of experimental design. d Expression of Drd1 in synaptosomes from control (Fold change D1R/Gapdh: 100 ± 17.57% relative to control; n = 5 mice) and Drd1-sgRNA KO striatum mice (Fold change D1R/Gapdh: 37 ± 9.03% relative to control; n = 5 mice) (t-test; t8 = 3.20, *p < 0.05)

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Molecular Brain

ISSN: 1756-6606