Fig. 2

Colocalization of Retromers with Either Early or Late Endosomes. a, b HeLa cells cotransfected with fluorescently labeled VPS29-YFP (green; retromers) and either RFP-Rab5a (red; early endosomes) or RFP-Rab7a (red; late endosomes) were imaged by time-lapse fluorescence microscopy. The pictures in this figure were extracted from Additional file 5: Movies S1 and Additional file 7: Movie S3. Vesicles positive for VPS29 were almost always found adjacent to Rab5a-positive vesicles. VPS29-positive vesicles were almost always double positive for Rab7a-positive vesicles. Yellow indicates the overlapping localization of green and red signals. c Immunostaining of iPSC-derived neurons (iNeurons) for endogenous VPS35 and either Rab5 or Rab7; Rab5 and Rab7 partially colocalized with VPS35. There was no significant difference between the PD and control groups. Scale bars, 10 μm in a and b and 5 μm in c. See also Additional file 4: Figure S3 and Additional file 5: Movies S1, Additional file 6: Movie S2, Additional file 7: Movie S3, Additional file 8: Movie S4