Fig. 2

Trafficking of exon 2a-GFP, exon 2b-GFP, exon 2c-GFP, and exon 6-GFP transcripts in hippocampal pyramidal neurons. a–d Densitometric analysis on in situ hybridization of apical dendrites of pyramidal neurons transfected with exon 2a-GFP (a), exon 2b-GFP (b), exon 2c-GFP (c) and exon 6-GFP (d) untreated (gray boxes) or treated with 10 mM KCl for 3 h (white boxes). Below each graph, representative straightened neurons labeled by in situ hybridization are reported. Lines in graphs represent the plot of the median gray value of GFP densitometric analysis untreated (solid line) and after KCl stimulation (dashed line). Scale bars: 10 μm. *p ≤ 0.05 exon-GFP ctrl respect to GFP control and exon-GFP KCl vs GFP KCl