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Fig. 3 | Molecular Brain

Fig. 3

From: Brain transcriptome analysis reveals subtle effects on mitochondrial function and iron homeostasis of mutations in the SORL1 gene implicated in early onset familial Alzheimer’s disease

Fig. 3

Allele-specific expression of sorl1 transcripts. a The number of copies of sorl1 transcripts present in +/+ (n = 3) and EOfAD/+ mutant (n = 3) brains per 50 ng of brain-derived cDNA. Data is presented as the mean ± SEM. In the +/+ brains, there were approximately 1400 copies of the wild type (WT) sorl1 transcript and no copies of the EOfAD-like (V1482Afs) allele transcript. In the EOfAD-like/+ brains, there were approximately 440 copies of the WT sorl1 transcript, and approximately 80 copies of the EOfAD-like allele transcript. P-values were determined by a one-way ANOVA with Dunnett’s T3 post-hoc test. b A schematic of sorl1 mRNA from exons 1–3. Primer binding sites are indicated by orange arrows, and the mutation site by the red star. Below is an image captured after agarose gel electrophoresis of the RT-PCR products. Lanes 1–3 are amplifications from three +/+ brains, while lanes 4–6 are amplifications from three null/+ brains. All fish were siblings

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Molecular Brain

ISSN: 1756-6606