Fig. 5

Hv1^−/− mice show reduced oxidative stress induced damage to neurons. a Bar graph shows ROS content in spinal tissue homogenates measured by the OxiSelect™ ROS/RNS assay kit in the sham and SCI groups in WT (gray) and Hv1^−/− (blue) mice. b Analysis of number of NeuN⁺ cells per field of view (fov) in the ventral horn of the spinal cord in sham, 1 d, 3 d and 7 d after injury. c Representative images show time course of neuronal loss (NeuN) in Hv1^−/− and WT mice after SCI (Scale: 200 µm). Data is represented as mean ± SEM. (*P < 0.05, **P < 0.01, ***P < 0.001, Student’s t-test, comparison between WT and Hv1^−/− for each time point, n = 3 per group/ time point)