Fig. 1

Leptin controls chloride homeostasis in rat hippocampal slices. a Current–voltage (I–V) relationships for evoked GABAergic synaptic currents before and during leptin application (100 nM, 20 min) a1 and in control experiments a2 during which neurons were recorded following the same protocol but leptin was omitted. The intercepts of the linear regression of the I–V curves was used to calculate E_GABA changes induced by leptin (∆E_GABA). Insets depict the GABAergic synaptic currents. Scale bars, 10 ms, 20 pA. b Box plots of ∆E_GABA induced by leptin 100 and 20 nM applied during 20 min. In control experiments (Ctr), neurons were recorded following the same protocol in the absence of leptin. c Loose patch recordings of CA3 pyramidal neurons on acute hippocampal slices before (− 10–0 min) and during (15–20 min) the application of leptin (100 nM, 20 min) and in control experiment, during which neurons recorded following the same protocol in the absence of leptin. Scale bar, 2 min, 50pA. d Box plots of leptin action on spike activity in the indicated conditions. ^##P < 0.01 when compared to pre-leptin values, two-tailed Wilcoxon paired test. *P < 0.05 when compared to leptin experiments, two-tailed Mann Whitney test