Fig. 4
From: Translational changes induced by acute sleep deprivation uncovered by TRAP-Seq
Many genes are regulated by sleep deprivation in the transcriptome but not in the translatome. a Venn diagram showing overlap of differentially expressed genes between TRAP and microarray after SD. b Quadrant scatter plots showing the fold change (log2) of DEGs exclusive from microarray with their respective fold change from TRAP (genes that are only responsive at the total RNA level but not at the level of ribosome-associated transcripts). The genes in the outermost layer are labeled with absolute delta fold change (FCmicroarray − FCTRAP) value above 0.4. c, d q-PCR validation of some of the genes associated with transcriptional repression after SD in males (c) and females (d). Unpaired t-test comparing SD vs NSD, males: Zmym1: p = ns, Hspb1: p = ns, Eif4e2: p = ns and Sin3b: p = ns, females: Zmym1: p = ns, Hspb1: p = ns, Eif4e2: p = ns and Sin3b: p = ns