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Fig. 4 | Molecular Brain

Fig. 4

From: Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice

Fig. 4

Characterization of Gad1tTA/STOP−tetO mice in the presence of Dox treatment. a The survival rates of adult Gad1+/+ (+/+) and Gad1tTA/STOP−tetO (tTA/tet) mice after starting Dox treatment. bd Representative western blot analysis of the expression of GAD65/67 and β-actin proteins in the frontal cortex (b), the hippocampus (c) and the cerebellum (d) of Gad1+/+ and Gad1tTA/STOP−tetO mice treated and not treated with Dox. β-Actin was used as a loading control. Three independent mice in the respective groups were examined. The band densities of GAD67 and β-actin were analyzed by ImageJ software. GAD67 protein levels were normalized to β-actin protein expression, and the % change was calculated relative to Gad1+/+ mice. The means with SE are demonstrated as columns. *p < 0.05 vs. the value of nontreated Gad1+/+ mice (Bonferroni test). #p < 0.05 vs. the value of nontreated Gad1tTA/STOP−tetO mice (Bonferroni test). e Representative immunoreactivities of GAD67 and parvalbumin (PV) proteins in the brain sections of nontreated Gad1+/+ mice, nontreated Gad1tTA/STOP−tetO mice and Dox-treated Gad1tTA/STOP−tetO mice. The white bars in the images indicate 2 mm length

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