High-brightness anterograde transneuronal HSV1 H129 tracer modified using a Trojan horse-like strategy

Table 2 In vivo gene copy number of exogenous gene in H1 or H8 infected mice brains were assessed using Q-PCR assay^a

Sample	UL37 quantity	UL37 adjusted quantity	egfp quantity	Adjusted relative ratio	Average ratio
H1	6,028,302.00	9,995,136.49	7,109,552.50	0.71	1.00 ± 0.16
H1	16,667,208.00	27,634,816.37	28,367,098.00	1.03
H1	2,208,835.25	3,662,326.44	4,622,589.50	1.26
H8	5,432,914.50	9,007,963.10	13,658,822.00	1.52	1.71 ± 0.10^b
H8	5,167,673.00	8,568,183.37	15,538,736.00	1.81
H8	7,823,168.50	12,971,088.20	23,258,302.00	1.79

^a For in vivo detection, 200 nl H1 and H8 virus was injected into midbrain VTA region of C57BL/6 mice, respectively. Three days after injection, mice brains were collected and ground evenly in liquid nitrogen with 1 ml lysis buffer. Then, 100 μl lysate of each brain was used to extract genome or protein. Gene copy number of GOI were determined by qPCR assay with the iQ SYBR Green Supermix kit (Bio-Rad). Results were expressed as means ± SEM of each group
^b Significant difference (p < 0.05), compared with H1 group

ISSN: 1756-6606