Fig. 2

Differential expression of miRNAs by toxicity-associated vs. synaptic stimulation. a QRT-PCR analysis of miRNA expression 4 h after stimulation with indicated drugs. BDNF (brain derived neurotrophic factor, 100 ng/ml), KCl (50 mM potassium chloride). Mean values (relative to untreated control) + SEM from ≥3 independent experiments are shown. P-values were determined with two-tailed t test. b, c Analysis of cell death induced by 4 h (B) or 10 min (C) treatment of neurons with KCl (50 mM) in the presence or absence of the NMDAR antagonist, MK-801 (10 μM). Mean values + SEM from three independent experiments are shown. P-values were determined with two-tailed paired t test (b) and repeated-measures ANOVA with Tukey’s post test (c). d QRT-PCR analysis of miRNA expression in 6 week old rats 4 h after intraperitoneal injection of kainate (10 mg/kg) or vehicle (PBS). Mean values + SEM are shown. N = 14 animals per group from four independent experiments. P-values were determined with two-tailed t test