Fig. 4

Drosha is required for NMDA-induced increases in TAM levels. a Representative Western Blot of hippocampal neurons that were left uninfected or that were infected with rAAV expressing scrambled or anti-Drosha shRNA. b Quantification of Western Blot experiments. DROSHA protein levels were calculated relative to uninfected controls. Mean + SEM from two independent experiments are shown. P-value was determined with two-tailed t test. Compared to uninfected and scrambled shRNA controls, targeting shRNA reduces DROSHA protein levels by approximately 60%. c, d QRT-PCR analysis of TAM and control miRNA expression in cells infected with rAAV expressing scrambled or anti-Drosha shRNA, without (basal, c) and with (induced, d) NMDA stimulation. Mean values (relative to uninfected control) + SEM from five independent experiments are shown. P-values were determined with two-tailed paired t test