Fig. 6
Postsynaptic and presynaptic upregulation of BKCa β4 subunit protein in the ACC during neuropathic pain associated anxiety-like behavior. a, Representative Western blots for PSD 95, syntaxin 1A, BKCa β4 and BKCa α subunits in the non-PSD and PSD membrane fractions of the ACC in sham and mCCD rats on postoperative day 14; b, BKCa β4 subunit was significantly enhanced in the PSD and non-PSD fractions of the ACC from mCCD (PSD fraction: 164 ± 37%, n = 6, *p < 0.05; non-PSD fraction: 224 ± 32%, n = 6, *p < 0.05) rats on postoperative day 14 compared with those in sham rats (PSD fraction: 100 ± 32%, n = 6; non-PSD fraction: 100 ± 12%, n = 6). BKCa α subunit showed no significant change in the PSD fraction between sham (100 ± 17%, n = 6) and mCCD (91.4 ± 14%, n = 6, p > 0.05) rats on postoperative day 14