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Table 1 Sequence of primers used for RT-PCR

From: Upregulation of Beta4 subunit of BKCa channels in the anterior cingulate cortex contributes to mechanical allodynia associated anxiety-like behaviors

GeneAccession no.Sequence of; F, forward primer; R, reverse primerRegionProduct
β-actinRatF:5′-ATGGTGGGTATGGGTCAGAAGG-3′208–472265 bp
 NM-031144R: 5′-TGGCTGGGGTGTTGAAGGTC-3’  
BKCaαsubunitRatF: 5′- CAGCACTCCGCAGACACTA-3’3774–3879106 bp
 NM-031828.1R:5′- ATCACCATAACAACCACCATCC-3’  
BKCaβ1 subunitRatF:5′-CGCCATCACTTACTACATCCT − 3’394–682289 bp
 019273.1R:5′-GACCTTCTTCACATCAACCAA-3’  
BKCaβ2 subunitRatF: 5′-CGGACCTCTTCATCTTACAGAC-3’22–210189 bp
 176,861.1R:5′-CAGCAGTGTGATTCCCAGTAG − 3’  
BKCaβ3 subunitRatF:5′-CGCTCTGATTGTTGGTTTGGT − 3’899–1059161 bp
 001104560.2R:5′-CTCTGTTCCTTCCCTGACTCTT-3’  
BKCaβ4 subunitRatF:5′-CATCCTGTCGCTCTTCATCTTC-3’102–335234 bp
 023960.2R: 5′ -TGGTGCTGGTCGCTGTGTA-3’  
  1. RT-PCR real-time polymerase chain reaction, BKCa large-conductance, calcium-activated potassium