Fig. 1
From: Proteomic analysis of synaptic protein turnover in the anterior cingulate cortex after nerve injury
Turnover rate of postsynaptic PKCγ in the ACC slows down after peripheral nerve injury. a Experimental schedule of proteomic analysis for examining the change of protein turnover rate. For adaptation mice were fed with 14N food pellets for 1 week before CPN ligation. Subsequently the diet was switched to 15N food pellets. The ACC and hippocampus were dissected 1 week after 15N diet consumption and used for LC-MS analysis. b SDS electrophoresis gel images. Red dotted boxes indicate proteins smaller than 90 kDa. Left; Sham group, Right; Nerve injury group. c Densitometric analysis of Coomassie blue-stained gels. Red dot lines indicate smaller than 90 kDa. d and e Volcano plots of fold change of % labeled peptide fraction (LPF) for each protein in the ACC (d) and hippocampus (e). Proteins with -log10 (p value) > 1.301 were considered significant. (n = 3 or 4 mice per group, unpaired t-test). KPCG; protein kinase C gamma. CH60; mitochondrial 60 kDa heat shock protein (see also Additional file 1: Table S1). f PKCγ levels in total and PSD fraction of the ACC show a opposite change after peripheral nerve injury. Pan-cadherin (pan-cad) and β-actin were used for loading control. Upper; Representative western blot image, Lower; Quantitative analysis of Western blot image (PSD fraction, n = 12 per group, one-way ANOVA test followed by Bonferroni’s Multiple Comparison Test; F(3,44) = 2.915, p < 0.05; posttest, * p < 0.05, total fraction, n = 10~11 per group, one-way ANOVA test; F(3,37) = 2.098, p > 0.05). g PKCγ levels in the PSD fraction of the hippocampus do not show a significant alteration. Pan-cadherin was used for loading control. Upper; Representative Western blot image, Lower; Quantitative analysis of Western blot image (n = 10 per group, one-way ANOVA test; F(3,36) = 2.126, p > 0.05)