Fig. 1

Co-immunostaining of Olig2 and NG2 in adult mouse CNS. a Sagittal overview of Olig2 and NG2 expression in the mouse brain. b–m High magnification images showing the expression relationship between Olig2 and NG2 in 12 selected brain regions. Note that nearly all NG2⁺ cells are co-stained with Olig2 as indicated by white arrowheads, while a considerable number of Olig2⁺ cells do not have NG2 expression, as indicated by white arrows. In the HIP, about 76.1% of Olig2⁺ cells had NG2 co-expression, which is the highest among selected regions. n Coronal overview of the mouse SC, showing the co-immunostaining of Olig2 and NG2. o High magnification image showing the colocalization of Olig2 and NG2 in the SC of mouse. Note that almost all NG2⁺ cells were positive for Olig2 as in other brain regions, but only 8.3% of Olig2⁺ cells were positive for NG2. Scale bars: 1000 μm (a), 50 μm (b–m, o), 500 μm (n)