Fig. 4

GSK2334470, a chemical inhibitor of PDK1, promotes axon regeneration in vitro. a In vitro axon regeneration assays. Mouse embryonic DRG neurons were re-plated in GSK2334470 (1 μM or 5 μM as indicated) or a vehicle (control) containing culture medium, fixed, and immunostained with TUJ1 antibody. Scale bar, 100 μm. b Average length of the regenerating axons from (a) (Mean ± S.E.M.; n = 151, 156, and 161 cells for each condition; **p < 0.01 from a t-test). c Western blot analysis of protein lysates from control (0, vehicle only) or GSK2334470-treated embryonic DRG neuron cultures. Numbers indicate the relative intensities