Fig. 2

Characterization of dopaminergic neurons differentiated from TH-GFP iPSC lines. a The brightfield and fluorescent images at day 7 after reseeding the neurospheres demonstrated GFP expression in some of the differentiated neurons derived from TH-GFP iPSC lines. “PRKN” represents PRKN-mutated patient. Scale bar, 50 µm. b Flow cytometric analysis showed GFP-positive fractions in the TH-GFP iPSC-derived neurons against the original iPSC-derived neurons at day 9 after reseeding the neurospheres. “PRKN” represents PRKN-mutated patient. c Immunofluorescence staining for GFP and TH confirmed similar expression patterns among TH-GFP iPSC-derived neurons. “PRKN” represents PRKN-mutated patient. Scale bar, 20 µm. d Pie graphs showing quantitative analysis of the proportion of TH+GFP+ (yellow areas), TH−GFP+ (green areas), TH+GFP− , or TH−GFP− (white areas) cells in the total DAPI+ cells from the immunofluorescence images (n = 10 per iPSC line) in (c). There were no TH+GFP− cells derived from any TH-GFP iPSC lines. “PRKN” represents PRKN-mutated patient. Values are shown as the mean ± SEM