Fig. 2

Electrophysiological analysis for the excitability of WT and Tg2576 mice. Aa Representative traces in the current-clamp recording from the WT (blue) and Tg2576 (green) dentate GCs in response to prolonged (1 s) depolarizing current injection (200 pA). Ab Number of spike evoked during 1 s current injections with varying amplitudes (from 100 to 600 pA, 100 pA increment). At all amplitudes, the number of spikes is significantly higher in Tg2576 GCs (green closed circle, n = 14) compared to WT GCs (blue closed circle, n = 15). Mean ± S.E.M. **P < 0.01; *** P < 0.001 B Expanded view of overlaid 1st APs evoked by 300 pA current injection to show the shortening of AP onset in Tg2576 GCs compared to WT GCs. Mean ± S.E.M. *** P < 0.001 C The mean values of the resting membrane potential (WT: − 84.5 ± 1.3 mV, Tg2576: − 81.8 ± 1.0 mV), input resistance (WT: 161.6 ± 6.9 MΩ, Tg2576: 158.8 ± 9.5 MΩ), and rheobase (WT: 181.1 ± 6.7 pA, Tg2576: 173.5 ± 7.7) from the WT (n = 15) and Tg2576 GCs (n = 14). Mean ± S.E.M. N.S., not significantly different. Da Representative traces of current-clamp recordings from CA1 of WT (blue) and Tg2576 mice (green). A train of APs were evoked by 1 s depolarizing current pulse injection (100 pA). Db The F-I curve for CA1 from WT (n = 4) and Tg2576 (n = 4) mice