Fig. 5
From: Drosophila Graf regulates mushroom body β-axon extension and olfactory long-term memory
Graf is expressed in MB α/β neurons and acts cell autonomously to regulate β-lobe midline stopping. a, b Knocking down Graf expression specifically in MB α/β neurons induces midline crossing of β-lobe axons. a Confocal z-projections of c739-GAL4/ + and c739-GAL4/UAS-GrafRNAi adult brains stained with anti-FasII antibody. b Quantification of the β-lobe midline-crossing phenotype. c739-GAL4/ + , n = 20 brains; c739-GAL4/UAS-GrafRNAi, n = 28 brains. c Confocal z-projections of wild-type and Graf mutant MARCM clones labeled with UAS-mCD8-GFP driven by OK107-GAL4. Brains were co-stained with anti-FasII to label MB α and β lobes. Genotypes: hs-FLP,tubP-GAL80,FRT19A/FRT19A; UAS-mCD8-GFP/ + ; OK107-GAL4/ + (wild-type clones) and hs-FLP,tubP-GAL80,FRT19A/Graf1,FRT19A; UAS-mCD8-GFP/ + ; OK107-GAL4/ + (Graf1 clones). Note that Graf mutant β axons in a Graf1/ + heterozygous brain exceed the β-lobe region, further extending to the contralateral side. Arrowheads indicate the brain midline. Arrows indicate β-axons crossing the midline. Scale bars, 50 μm