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Fig. 1 | Molecular Brain

Fig. 1

From: Atg7 deficiency in microglia drives an altered transcriptomic profile associated with an impaired neuroinflammatory response

Fig. 1

Stable knockdown of Atg7 gene expression using small-hairpin RNA in BV2 microglia. a Immunoblot analysis of ATG7 protein expression levels in BV2 microglia cell lines infected with lentivirus expressing a shRNA targeting Atg7 expression (shAtg7 BV2 cells), or with a control scramble shRNA (shCtrl BV2 cells). The expression of ACTB as housekeeping gene was used as loading control. b The graphs show the quantification for ATG7 versus ACTB protein expression ratio in shAtg7 BV2 cells as compared to shCtrl BV2 cells used as control. c Comparison of Atg7 mRNA expression measured by RT-qPCR in shAtg7 BV2 cells and shCtrl BV2 cells. d Immunofluorescence analysis of ATG7 protein expression in shAtg7 BV2 cells and shCtrl BV2 cells. Chromosomal dye Hoechst is used for nuclear counterstaining. Phalloidin staining shows actin filaments (scale bar 50 µM). All values are a mean of three independent experiments ± SEM and considered significant for ****p < 0.0001

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Molecular Brain

ISSN: 1756-6606