Fig. 8

Microglial Atg7 deficiency is associated with reduced neurotoxicity capability but does not alter tumor migration supporting function. a Neurotoxicity of LPS-treated BV2 cells on dopaminergic MN9D neuronal cells was assessed by co-culturing the neurons with either shCtrl or shAtg7 cells and subsequentially treating them with LPS (100 ng/ml) for 24 h. Neurons, stained with a cell tracker, with abnormal nucleus morphology were counted and displayed in the graph as total cell death in percent for each co-culture condition. b Quantification of the migration of C6 glioma cells in transwells with shCtrl or shAtg7 BV2 microglia. Results are relative compared to the C6 migration in wells without BV2 cells. c Logarithmic display of fluorescence representing intracellular ROS in shCtrl and shAtg7 cells after 1 h of LPS (100 ng/ml) as compared to unstimulated. d Intracellular ROS in shCtrl and shAtg7 cells after 1 h of LPS (100 ng/ml). Values are a mean of 3 (a, b) or 4 (c) independent experiments ± SEM and considered significant for *p < 0.05, **p < 0.01. n.s., not significant for the indicated comparison. c, d Chi square test is shown as statistical value to compare the cell populations in both graphs