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Table 2 List of RT-qPCR primers

From: Atg7 deficiency in microglia drives an altered transcriptomic profile associated with an impaired neuroinflammatory response

Genes

Forward primer

Reverse primer

RT-qPCR primers

 Actb

GATGTATGAAGGCTTTGGTC

TGTGCACTTTTATTGGTCTC

 Apoe

ACCTGATGGAGAAGATACAG

GATATGGATGTTGTTGCAGG

 Arg1

GGAGACCACAGTCTGGCAGTTGGA

GGACACAGGTTGCCCATGC

 Atg7

CTGTTCACCCAAAGTTCTTG

TCTAAGAAGGAATGTGAGGAG

 Nfkb1

AGGACATGGGATTTCAGGA

GGAGGTGGATGATGGCTAA

 Nfkb2

TCAAGATCTGTAACTATGAGGG

TTCTTCTTGGTTACATGCAG

 Nos2

GCGGAGTGACGGCAAACAT

AGGTCGATGCACAACTGGG

 Rel

ATACCTGCCAGATGAAAAAG

TCAGTAAAGTGACCACAATC

 Rela

GCTCCTAAGGTGCTGACA

ACCTCCGAAAGCGAGATA

 Relb

CGGATTTGCCGAATCAAC

CGGATATGTCCTCTTTTTGC

  1. List of primer sequences used in this study. All the sequences are given 5ʹ to 3ʹ
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Molecular Brain

ISSN: 1756-6606