Fig. 1

Decreased GABAergic synaptic responses in hippocampal CA1 neurons of IN-PS cDKO mice. a Representative spontaneous inhibitory postsynaptic currents (sIPSCs) recorded in CA1 pyramidal neurons from control and IN-PS cDKO mice at a holding potential of − 70 mV in the presence of blockers of AMPA (10 µM NBQX) and NMDA (50 µM APV) receptors. b Cumulative inter-event interval (Control: 2033 events in 12 slices, cDKO: 2791 events in 13 slices) and amplitude (Control: 291 events in 12 slices, cDKO: 483 events in 13 slices) histograms of sIPSCs recorded in slices from control and IN-PS cDKO mice, showing a reduction in sIPSC frequency but no change in sIPSC amplitude from IN-PS cDKO mice. c Statistical analysis indicates that IN-PS cDKO neurons exhibit significantly reduced sIPSC frequency (Control: 4.39 ± 0.25 Hz, cDKO: 2.86 ± 0.27 Hz; p = 0.0004, unpaired t-test) but unchanged amplitude (Control: 63.3 ± 1.9 pA, cDKO: 59.0 ± 2.7 pA; p = 0.22, unpaired t-test). d Schematic drawing shows mono-synaptic fast GABAergic inhibition of CA1 pyramidal neurons. e Input/output relations of evoked GABA_AR IPSCs in slices from control and IN-PS cDKO mice. The evoked IPSCs were elicited by the stimulation electrode placed close to the recording electrode in the CA1 stratum pyramidale and recorded at a holding potential of − 70 mV with Cs⁺-based pipette solution containing high [Cl⁻]. The IPSC amplitude is plotted as a function of stimulation intensity. IN-PS cDKO neurons show reduction of evoked IPSC amplitudes (F_{1, 22} = 5.02, p = 0.03, two-way ANOVA). All data represent mean ± SEM (* p < 0.05, *** p < 0.001). The number of neurons/mice used in each experiment is shown in parentheses