Fig. 3
From: Reversibility of motor dysfunction in the rat model of NGLY1 deficiency
Distribution of hNGLY1 protein in brains after intracerebroventricular injection of AAV9-hNGLY1 in Ngly1−/− rats. a–c Nuclei were stained with DAPI (blue). Green or red signals indicate hNGLY1-positive cells. a, b Representative sagittal (a) and coronal (b) brain sections from 11-week-old AAV-injected Ngly1−/− rats. Scale bar = 2.5 mm. c Higher magnification images of NGLY1-positive cells across various brain regions after i.c.v. administration. Gray scale bar = 0.25 mm, white scale bar = 0.1 mm. d Immunohistochemical staining with hNGLY1 (red) and glial or neuronal markers (green) in the brains of 11-week old AAV-injected rats, using antibodies to Ngly1, GFAP (a marker for astrocytes), IbaI (a marker for microglia), GSTP1 (a marker for oligodendrocytes), and NeuN (a marker for mature neurons). Nuclei have been stained with DAPI (blue). Scale bar = 50 μm