Fig. 4

PCDH19 as a potential candidate gene regulated by Bmal1. A The volcano plot of differentially expressed genes between the control group and the cKO group (FDR < 0.05). B Heat map of significantly up- or down-regulated genes between the control group and the cKO group. Each group contained three batches of individual samples. C The number of the significantly differentially expressed genes between the control group and the cKO group (fold change > 2, FDR < 0.05). D, E Immunofluorescence detection of mCherry and PCDH19 expression in DG of mice. Scale bar, 50 μm. Analysis of fluorescence intensity was performed using ImageJ. Differences in the intensity between the control group and the cKO group were analyzed with the Students t-test (Control: 1.000 ± 0.049, cKO: 0.592 ± 0.052, n = 3, p = 0.007). F, G PCDH19 expression was significantly decreased at the cKO group, compared with the Ctrl group. Differences in the levels of PCDH19 are presented as mean ± SEM and analyzed using unpaired Student’s t-test (control: 1.003 ± 0.131, cKO: 0.330 ± 0.033, n = 3, p = 0.008). The data are expressed as mean ± SEM and analyzed with unpaired Student’s t-test. *p < 0.05, **p < 0.01