Fig. 1

Syph Ct contains repeated regions and alone forms liquid droplets when incubated at high concentrations in vitro. a Domain structure of full-length mouse Syph. Nt N-terminus, TM transmembrane domain, Ct C-terminus (amino acids 219–308). b Repeated sequence in the cytoplasmic domain synaptophysin Ct. Syph Ct contains 10 repeated regions, 9 of which start with tyrosine. The repeated sequences are aligned to show the consensus sequence Y-G-P/Q-Q-G. c The pie graph shows the proportion of Tyr, Gly, Pro and Gln residue in the Syph Ct. d The prediction plot of intrinsically disordered regions in the full-length Syph using PrDOS. The shaded region is Syph Ct, which is likely to be an IDR. e Fluorescence images showing droplet formation of purified Syph Ct-mCh alone (50 μM) in vitro in the presence of 10% PEG-8000 at RT. f Representative fluorescence image of Syph Ct-mCh expressed in COS-7 cells. Scale bars, 20 μm