Fig. 6
Chemogenetic activation of GABAergic neurons in the ACC decreased the excitability of ACC PNs. A Representative images of c-Fos in the contralateral ACC of Model-mCherry-CNO and Model-hM3D(Gq)-CNO group rats. The number of C-Fos cells per 0.1mm2 in the contralateral ACC of Model-mCherry-CNO and Model-hM3D(Gq)-CNO group rats. n = 3–4. **P < 0.01, Model-hM3D(Gq) group vs Model-hM3D(Gq)-CNO group. Bar = 50 μm. B, C The firing rates in response to prolonged depolarizing current injections of increasing amplitudes in different groups. D, F The firing phenotypes changes of CNO application of PNs in ACC layer V. The input resistance (H, J), AP threshold (E, G) and membrane potentials (I, K) of PNs in ACC layer V were statistically analyzed in each group. n = 10 neurons from 4 rat/group. **P < 0.01, Model-hM3D(Gq) group vs Model-hM3D(Gq)-CNO group. All data represent the mean ± SEM