Fig. 4

Effect of an H3K27 demethylase inhibitor on electrocautery-induced allodynia. A Schematic diagram of the mechanism of action of GSK-J4. B Protocol for measurement of the pain threshold and drug administration. C Changes in the pain threshold as measured by the von Frey test (0.16 g). Each point represents the mean ± S.E.M. of 5 mice (Two-way repeated measures ANOVA with post-hoc Bonferroni test, *p < 0.05, **p < 0.01, ***p < 0.001 vs. Sham-Vehicle group, ^#p < 0.05 vs. Electrocautery-Vehicle group). D, E The mRNA levels of Sprr1a D or Anxa10 E in the ipsilateral side of the spinal cord were measured at 18 days after surgery. Each column represents the mean ± S.E.M. of 5 independent experiments (One-way ANOVA with post-hoc Bonferroni test, *p < 0.05, ***p < 0.001 vs. Sham-Vehicle group, ^#p < 0.05, ^###p < 0.001 vs. Electrocautery-Vehicle group)