Fig. 12

Overexpression of lipocalin 2 in the brain alters stress-related gene expression in the hippocampus. After a single intracerebroventricular injection of recombinant lipocalin 2 protein (7 μL, 1 μg/mL) or vehicle in the brain of the mice for 7 days, the hippocampal tissues were collected for mRNA assay. The expression of MR, GR, MAO-A, or MAO-B was determined by real-time PCR. Quantitative data are presented as mean ± SEM (n = 5 per group). The Student’s t-test was used to examine the significance of the mean. * p < 0.05 vs. the vehicle group. ** p < 0.01 vs. the vehicle group. **** p < 0.001 vs. the vehicle group. GR: glucocorticoid receptor, MAO-A: monoamine oxidase A, MAO-B: monoamine oxidase B, MR: mineralocorticoid receptor, PCR: polymerase chain reaction, SEM: standard error of mean