Fig. 2
From: Ackr3-Venus knock-in mouse lights up brain vasculature

Ligand profiling of βarrestin2 recruitment to ACKR3-Venus in HEK-293 cells. A Schematic representation of the BRET1 biosensor monitoring the interaction between βarrestin2-RlucII (BRET1 sensor donor) and Venus-tagged ACKR3 (BRET1 sensor acceptor) [79]. B Cells transfected with ACKR3-Venus and βarrestin2-RlucII were exposed for 10 min to increasing concentrations of the indicated compound. Data are expressed as % of CXCL12 maximum response (Mean ± SEM) of 3–5 independent experiments