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Fig. 3 | Molecular Brain

Fig. 3

From: Ackr3-Venus knock-in mouse lights up brain vasculature

Fig. 3

Ackr3-Venus knock-in mouse generation. A The scheme shows the strategy used to generate Ackr3-Venus knock-in mice (see methods). Briefly, the intron 1- exon 2 of Ackr3 was replaced with a targeted fragment containing Ackr3-fused to Venus at C-tail. LoxP sites are shown by black flags, closed box is 3′ UTR. BE Evaluation of Ackr3-Venus transcript levels from RNA extracts of whole mouse brain. Real-time qPCR results show expression levels of the following transcripts Ackr3 (B), Venus (C) mGluR5 (D), and β-actin (E), in Ackr3WT/WT, Ackr3Venus/WT, and Ackr3Venus/Venus (n = 3–6 animals per group). The ΔΔCT in percent of WT is shown for each transcript except Venus where KI was used for normalization. Significance is shown as *p < 0.05 and ***p < 0.001 in a one-way ANOVA with Dunn’s multiple comparison’s test

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