Fig. 2

Pain and anxiety symptoms assessment of three PD mice models. a–d Mechanical threshold were reduced (a) and mechanical allodynia were increased (b) in all three PD models. Response latency in hot plate test was reduced in A53T transgenic and MPTP-treated mice (c). MPTP-treated and 6-OHDA-treated mice exhibited shorter response latency in tail flick test (d). e–h Representative traces showing the movement of mice in EPM (e) and open field test (h). f–i MPTP-treated and 6-OHDA-treated mice exhibited increased anxiety-related behaviors in EPM (f) and open field test (i), and there was no significant difference of entry number in EPM (g). (n = 10 in WT and A53T groups, n = 10 in saline and MPTP groups, n = 9 in saline and 6-OHDA groups) (In hot plate test, tail flick test, open field test and EPM, n = 12 in WT and A53T groups, n = 13 in saline and MPTP groups, n = 12 in saline and 6-OHDA groups. In mechanical threshold test, n = 6 in WT and A53T groups, n = 6 in saline and MPTP groups, n = 6 in saline and 6-OHDA groups. In mechanical allodynia test, n = 6 in WT and A53T groups, n = 7 in saline and MPTP groups, n = 6 in saline and 6-OHDA groups) *p < 0.05, **p < 0.01